Composite

Part:BBa_K3182300

Designed by: Oliver Hild Walett   Group: iGEM19_Linkoping_Sweden   (2019-07-15)
Revision as of 13:17, 18 September 2019 by Olle111 (Talk | contribs)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

pT7-mNG

This is a improved variant of BBa_K2671000. This part codes for mNeonGreen, which is a fluorescent protein with great intensity. mNeonGreen has a excitation maximum at 506 nm and a emission maximum at 517, providing a small Stoke's shift. It's color can be seen under normal white light as a yellow-green color. This part includes a T7 promotor as well as a 5'-UTR region for higher expression.


Figure 1. A schematic representation of the improvement. Where the promotor (small green arrow) has been changed from pBAD --> pT7. Extra bases which are non-coding (black line behind mNeonGreen in upper part) was also removed.

























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