Part:BBa_K2992027:Design

Design Considerations

Amplified unmodified from the genome sequences of the host organisms. The bgaRL regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the bgaRL system many constituents are listed in the 3’-5’-orientation to allow correct topology when applied to composite parts.