Regulatory

Part:BBa_K2992022:Design

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-17)
Revision as of 12:52, 17 September 2019 by YasTen (Talk | contribs) (→‎Design Notes)


5-UTR containing RBS for bgaR from C. perfringens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Unmodified from C. perfringens genome sequence. This regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system.This regulator forms part of lactose inducible unit and is therefore listed in the 3’-5’-orientation to allow correct topology when applied to composite parts.

Source

C. perfringens genomic DNA

References