Composite

Part:BBa_K2959004:Design

Designed by: Alejandro Aguirre Hernndez   Group: iGEM19_Tec-Chihuahua   (2019-09-16)
Revision as of 02:28, 17 September 2019 by Alejandro ah (Talk | contribs)


Expressible Pinus sylvestris Defensin 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of PsDef1 is preceded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.


Source

The composite part was designed using the following parts previously added to the Registry: BBa_R0010, BBa_B0034, and BBa_B0015. The amino acid sequence for PsDef1 was retrieved from Kovalyova et al. (2007).


References

Kovalyova, V. A., Gout, I. T., Kyamova, R. G., Filonenko, V. V., & Gout, R. T. (2007). Molecular cloning and characterization of defensin 1 from Scots pine. Biopolymers and Cell,23(5), 398-404.