Generator

Part:BBa_K3174004

Designed by: iGEM19_Austin_UTexas   Group: iGEM19_Austin_UTexas   (2019-08-08)
Revision as of 17:18, 16 September 2019 by Dbhat (Talk | contribs)


Blue Fluorescent Protein with Strong Promoter and Weak RBS

This part has the blue fluorescent protein (mTagBFP) gene inserted downstream of a strong promoter and weak ribosome binding site.

BBa_J23104 is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley.

BBa_B0031 is an RBS based on the Elowitz repressilator and was designed in 2003. Its relative strength was characterized by IIT Madras in 2016 and by Team Warsaw in 2010.

BBa_K592100 is a blue fluorescent protein designed by iGEM11_Uppsala-Sweden in 2011. It has a maximum emission of 456 nm.


Usage and Biology

UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor (https://2019.igem.org/Team:Austin_UTexas) and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other biobricks that had been similarly transformed into the burden strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None