Part:BBa_K3204001
C-LytAm7 Affinity Polypeptide Tag
The C-LytA protein constitutes the choline-binding module of the LytA amidase from Streptococcus pneumoniae. Owing to its affinity for choline and analogs, it is regularly used as an affinity tag for the purification of proteins in a single chromatographic step.
The affinity of the C-LytA module for choline and structural analogues, has allowed the development of an efficient system for the overexpression and purification of fusion proteins tagged to C-LytA using commercial resins such as diethylaminoethanol (DEAE) cellulose or even aqueous two-phase systems, and that has been commercialized (http://www.biomedal.com). Moreover, the crystal structure of the protein has been solved and its folding and stability have been investigated and modified by protein engineering. Besides, the use of relatively sizeable tags such as C-LytA may help the immobilized protein of interest to avoid steric hindrances with the support and to increase its mobility.
The C-LytA tag can also be used to immobilize protein onto gold or graphite electrodes to perform electrochemical measurments.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |