Coding

Part:BBa_K2933018

Designed by: Yongjie Li   Group: iGEM19_TJUSLS_China   (2019-09-08)
Revision as of 11:26, 16 September 2019 by Yongjie (Talk | contribs)


subclass A metallo-beta-lactamase ARL-1, codon optimized in E. coli

This part encodes a protein called ARL-1, which is a beta-lactamase of subclass A.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 427
    Illegal PstI site found at 541
    Illegal PstI site found at 592
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 427
    Illegal PstI site found at 541
    Illegal PstI site found at 592
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 427
    Illegal PstI site found at 541
    Illegal PstI site found at 592
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 427
    Illegal PstI site found at 541
    Illegal PstI site found at 592
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

ARL-1 is a type of subclass A beta-lactamases, which is separated from Staphylococcus arlettae. The blaARL-1 was recently discovered and reported, that have a high mutation.

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Categories
Parameters
None