Coding

Part:BBa_K2959002:Design

Designed by: Alejandro Aguirre Hernndez   Group: iGEM19_Tec-Chihuahua   (2019-09-15)
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Truncated Saccharomyces cerevisiae FAD-linked sulfhydryl oxidase Erv1p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 58
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence consists in a truncated version of Erv1p that lacks the first 72 amino acids of the full sequence. It has been proved that this version shows similar or even improved sulfhydryl oxidase activity.


Source

The full amino acid sequence was reported by Lisowsky (1996). The truncated Erv1p amino acid sequence was retrieved from Lee et al. (2000).

References