Coding

Part:BBa_K2933134

Designed by: Wenhui Gong   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 13:50, 15 September 2019 by Registry (Talk | contribs)

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His+Linker a+Sumo+Linker b+TMB-2

This part encodes the fusion protein of His tag, sumo tag and TMB-2 to promote the expression and purification of target protein(TMB-2).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
    Illegal NheI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
    Illegal BglII site found at 145
    Illegal BamHI site found at 344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None