Composite

Part:BBa_K2933281

Designed by: Weisi Wang   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 11:08, 15 September 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


RBS+Linker h+His+Linker f+ARL-1+T7 terminator

This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+ARL-1) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 555
    Illegal PstI site found at 669
    Illegal PstI site found at 720
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 985
    Illegal PstI site found at 555
    Illegal PstI site found at 669
    Illegal PstI site found at 720
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 555
    Illegal PstI site found at 669
    Illegal PstI site found at 720
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 555
    Illegal PstI site found at 669
    Illegal PstI site found at 720
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None