Composite

Part:BBa_K2933280

Designed by: Weisi Wang   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 11:06, 15 September 2019 by Registry (Talk | contribs)

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RBS+Linker h+His+Linker f+PST-1+T7 terminator

This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+PST-1) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 865
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None