Composite

Part:BBa_K2933275

Designed by: Weisi Wang   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 10:51, 15 September 2019 by Registry (Talk | contribs)

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RBS+Linker h+His+Linker f+JOHN-1+T7 terminator

This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+JOHN-1) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 204
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 883
    Illegal PstI site found at 204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 204
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 204
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 728


[edit]
Categories
Parameters
None