![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2933275
Designed by: Weisi Wang Group: iGEM19_TJUSLS_China (2019-09-15)
RBS+Linker h+His+Linker f+JOHN-1+T7 terminator
This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+JOHN-1) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 204
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 883
Illegal PstI site found at 204 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 204
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 204
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 728
[edit]
Categories
Parameters
None |