Composite
Part:BBa_K2933272
Designed by: Weisi Wang Group: iGEM19_TJUSLS_China (2019-09-15)
RBS+Linker h+His+Linker f+CPS-1+T7 terminator
This part consists of RBS, protein coding sequence(His+Linker h+Sumo+Linker f+CPS-1) and T7 terminator,and the biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 665
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 1009
Illegal PstI site found at 665 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 947
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 665
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 665
Illegal AgeI site found at 566 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |