Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K2933242

Designed by: Yongjie Li   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 09:33, 15 September 2019 by Registry (Talk | contribs)

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RBS a+Linker g+GST+Linker e+Fla.103

This part consists of RBS a, protein coding sequence(GST+Linker e+Fla.103). The blaFla.103 is separated from Flavobacterium sp. 103. The RBS and the protein coding sequence can be connected by linker g. The biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 802
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 802
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1284
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 802
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 802
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 113


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Parameters
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