Composite

Part:BBa_K2933235

Designed by: Yongjie Li   Group: iGEM19_TJUSLS_China   (2019-09-15)
Revision as of 09:17, 15 September 2019 by Registry (Talk | contribs)

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RBS a+Linker g+GST+Linker e+BcII-194

This part consists of RBS a, protein coding sequence(GST+Linker e+Bcll-194), the RBS and the protein coding sequence can be connected by linker g. The blaBcll-194 is a metal β-lactamase which comes from Bcll family. The biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 113


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Categories
Parameters
None