Composite
Part:BBa_K2933225
Designed by: Yongjie Li Group: iGEM19_TJUSLS_China (2019-09-15)
RBS a+Linker g+GST+Linker e+ElBlaII
This part consists of RBS a, protein coding sequence(GST+Linker e+ElBla2-1), the RBS and the protein coding sequence can be connected by linker g. ElBla2-1 is a MBLs isolated from B that we temporarily named it as ElBla2-1. The biological module can be build into E.coli for protein expression. This part can be prefaced with promoters of different strengths and types to regulate expression function.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1210
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 113
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Categories
Parameters
None |