Composite
Part:BBa_K2933204
Designed by: Pengqian Li Group: iGEM19_TJUSLS_China (2019-09-14)
T7 promoter+RBS b+Linker h+His+Linker f+SPG-1+T7 terminator
This part consists of T7 promoter, RBS and protein coding sequence(His+Linker f+SPG-1),and the biological module can be built into E.coli for protein expression
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 169
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |