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Part:BBa_K3063020:Design

Designed by: Ng Tsz Chun   Group: iGEM19_Hong_Kong_HKU   (2019-09-14)
Revision as of 17:51, 14 September 2019 by Registry (Talk | contribs)

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Strand 2 (free aptamer) for in vivo synthesis of DNA nanostructure


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 57
    Illegal BsaI.rc site found at 39


Design Notes

The design also allow strand 1 without aptamer to be produced. For the production of strand without any aptamer attached, use 5' phosphorylated primers complementary to the BsaI produced sticky end. Anneal (high ratio of primers), ligate and do a blunt end ligation.


Source

The DNA nanostructure sequences were produced in computer software (Tiamat).

References