Coding

Part:BBa_K2933127

Designed by: Wenhui Gong   Group: iGEM19_TJUSLS_China   (2019-09-14)
Revision as of 14:22, 14 September 2019 by Registry (Talk | contribs)

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His+Linker a+Sumo+Linker b+PEDO-1

This part encodes the fusion protein of His tag, sumo tag and PEDO-1 to promote the expression and purification of target protein(PEDO-1).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
    Illegal PstI site found at 892
    Illegal PstI site found at 1178
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
    Illegal NheI site found at 33
    Illegal PstI site found at 892
    Illegal PstI site found at 1178
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
    Illegal BglII site found at 145
    Illegal BamHI site found at 344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
    Illegal PstI site found at 892
    Illegal PstI site found at 1178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
    Illegal PstI site found at 892
    Illegal PstI site found at 1178
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None