![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2933127
Designed by: Wenhui Gong Group: iGEM19_TJUSLS_China (2019-09-14)
His+Linker a+Sumo+Linker b+PEDO-1
This part encodes the fusion protein of His tag, sumo tag and PEDO-1 to promote the expression and purification of target protein(PEDO-1).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 256
Illegal PstI site found at 892
Illegal PstI site found at 1178 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 256
Illegal NheI site found at 33
Illegal PstI site found at 892
Illegal PstI site found at 1178 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 256
Illegal BglII site found at 145
Illegal BamHI site found at 344 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 256
Illegal PstI site found at 892
Illegal PstI site found at 1178 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 256
Illegal PstI site found at 892
Illegal PstI site found at 1178 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |