Composite
Part:BBa_K2933171
Designed by: Weisi Wang Group: iGEM19_TJUSLS_China (2019-09-14)
Tac promoter+RBS a+Linker g+GST+Linker e+JOHN-1
This part consists of Tac promoter,RBS and protein coding sequence (GST+Linker e+JOHN-1),and the biological module can be built into E.coli for protein expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 870
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 870
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 870
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 870
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 181
Illegal SapI.rc site found at 1394
[edit]
Categories
Parameters
None |