Coding

Part:BBa_K2933103

Designed by: Dongxu Li   Group: iGEM19_TJUSLS_China   (2019-09-06)
Revision as of 03:38, 14 September 2019 by Wenhui (Talk | contribs)


His+Linker a+Sumo+Linker b+ElBlaII

This part encodes the fusion protein of His-Sumo tag and ElBla to promote the expression and purification of target protein(ElBla2-1).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
    Illegal NheI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
    Illegal BglII site found at 145
    Illegal BamHI site found at 344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
    Illegal AgeI site found at 839
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None