Composite

Part:BBa_K2963029

Designed by: Experiment group advisor of JNU-China   Group: iGEM19_JNU-China   (2019-09-12)
Revision as of 17:14, 12 September 2019 by Registry (Talk | contribs)

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BsracE18000 - Bicistronic containing RBS18000 to stabilize gene expression.

According to that pre-cistron can affect the expression of next cistron, and we select a short 5' sequence of high-protein-abundance gene from Corynebacterium glutamicum as the pre-cistron trying to improve the stabilization of the expression of racE(coding racemase enzyme).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 994
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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