Coding
Part:BBa_K3168003
Designed by: Eva Hanckmann, Harm van der Veer, Claire Michielsen Group: iGEM19_TU_Eindhoven (2019-09-12)
SmallBitNanoLuc
This basic part codes for the small bit of NanoLuc, which can be used as a split reporter. In combination with the large bit of NanoLuc and the addition of furimazine (the substrate) bright blue light is emitted. A flexible (GGS)5 linker is located in front of the small bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 85
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |