Composite

Part:BBa_K2963021

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-12)
Revision as of 08:34, 12 September 2019 by Registry (Talk | contribs)

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pgsBCA- encoding a poly-γ-glutamic acid synthetase

PgsBCA complex, consisting of three subunits, is composed of PgsB、PgsC and PgsA. PgsB catalyzes poly-gamma-Glutamic acid synthesis．And PgsC links PgsB and PgsA in the membrane. While PgsA transports poly-gamma-Glutamic acid outside the cell.In our project, we use the PgsBCA complex to biosynthesize poly-gamma-Glutamic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2207
    Illegal PstI site found at 2188
    Illegal PstI site found at 3467
    Illegal PstI site found at 3753
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2365
    Illegal NheI site found at 2963
    Illegal NheI site found at 4254
    Illegal PstI site found at 2188
    Illegal PstI site found at 3467
    Illegal PstI site found at 3753
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2207
    Illegal PstI site found at 2188
    Illegal PstI site found at 3467
    Illegal PstI site found at 3753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2207
    Illegal PstI site found at 2188
    Illegal PstI site found at 3467
    Illegal PstI site found at 3753
    Illegal NgoMIV site found at 2586
    Illegal AgeI site found at 4111
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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