Coding

Part:BBa_K3022002:Design

Designed by: Zhanyu Gao   Group: iGEM19_Nanjing-China   (2019-09-11)
Revision as of 07:50, 11 September 2019 by Registry (Talk | contribs)

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ppk1 in Citrobacter freundii ATCC 8090


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genomic DNA of Citrobacter f.reundii was used as the template to PCR-amplify ppk1 with PPKF and PPKR primers.


Source

Wild-type Citrobacter freundii ATCC 8090 was purchased from China Center of Industrial Culture Collection (CICC, China). For the construction of pBBR1MCS2-ppk1, genomic DNA of Citrobacter f.reundii was used as the template to PCR-amplify ppk1 with primers.

References