Part:BBa_K3145002:Experience

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Applications of BBa_K3145002

After we confirmed through sequencing that we removed the XbaI cut site between the T7 promoter and RBS region, we created six overnights that contained 3 mL of LB Broth, 3 µL of the antibiotic Kanamycin (50 ng/µL) and either E.coli DH5α cells containing T7mut-sfGFP (three overnights) or either E.coli DH5α cells containing pY71-sfGFP(three overnights) to compare the functionality between the two plasmids, and one overnight that only contained 3 mL of LB Broth only to serve as our blank. [[File:T7mutovernights.jpeg|400px|thumb|center|]

User Reviews

UNIQ9ddcfaf1ac524cfe-partinfo-00000000-QINU UNIQ9ddcfaf1ac524cfe-partinfo-00000001-QINU