Reporter

Part:BBa_K3056001

Designed by: Ruben Warkentin   Group: iGEM19_Queens_Canada   (2019-09-10)
Revision as of 23:35, 10 September 2019 by Registry (Talk | contribs)

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Anti-THC antibody fragment (ScFv), bound to EGFP at the C-terminal

Part encodes for an antibody fragment designed to detect delta-9-tetrahydrocannabinol (THC). The antibody fragment is linked to EGFP via a short GSGSGS linker on the C-terminal. The transcript is codon optimized for expression in E. coli. Additionally, an OmpA sequence proceeds the transcript, which serves as a per-plasmic localization signal. To function properly, the protein needs to form disulfide bonds, which it can only do in the oxidizing environment of the peri-plasmic environment. However, for best expression, we recommend Rosetta-gami2 for expression, which contains an oxidizing cytoplasmic environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1380
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 820


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