Regulatory
PR1 promot

Part:BBa_K2938000:Design

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-09-09)
Revision as of 12:38, 9 September 2019 by Registry (Talk | contribs)

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Attenuated pBEST-PR promoter after point mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None, this promoter was already in use in our lab.


Source

Schlesinger, Orr, et al. "Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates, and incorporation of noncanonical amino acids." ACS synthetic biology 6.6 (2017): 1076-1085. https://www.biorxiv.org/content/10.1101/100982v1.full


References