![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2933010
Designed by: Wenhui Gong Group: iGEM19_TJUSLS_China (2019-09-07)
subclass B1 metallo-beta-lactamase MUS-2, codon optimized in E. coli
This part encodes a protein called MUS-2, which is a metallo-beta-lactamase of subclass B2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
MUS-2 is 啊
Molecular cloning
We used the vector pGEX-6p-1 to construct our expression plasmid.
Figure 1. Left: The PCR result of MUS-2. Right: The verification results by enzyme digestion.
After verification, it was determined that the construction is successful.
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Categories
Parameters
None |