Coding

Part:BBa_K2933012

Designed by: Yongjie Li   Group: iGEM19_TJUSLS_China   (2019-09-08)
Revision as of 08:55, 8 September 2019 by Yongjie (Talk | contribs)


subclass B1 metallo-beta-lactamase JOHN-1, codon optimized in E. coli

This part encodes a protein called JOHN-1, which is a metallo-beta-lactamase of subclass B1.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 76
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 76
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 600


Usage and Biology

Flavobacterium johnsoniae (formerly Cytophaga johnsonae) is an environmental bacterium that can cause skin lesions in fish and is a plant pathogen.

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Categories
Parameters
None