Coding
Part:BBa_K2933012
Designed by: Yongjie Li Group: iGEM19_TJUSLS_China (2019-09-08)
subclass B1 metallo-beta-lactamase JOHN-1, codon optimized in E. coli
This part encodes a protein called JOHN-1, which is a metallo-beta-lactamase of subclass B1.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 76
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 76
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 76
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 76
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 600
Usage and Biology
Flavobacterium johnsoniae (formerly Cytophaga johnsonae) is an environmental bacterium that can cause skin lesions in fish and is a plant pathogen.
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Categories
Parameters
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