Composite

Part:BBa_K2963009:Design

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-08)
Revision as of 08:23, 8 September 2019 by Registry (Talk | contribs)

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capB*CA - encoding poly-γ-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
    Illegal NheI site found at 2365
    Illegal NheI site found at 2963
    Illegal NheI site found at 4281
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
    Illegal NgoMIV site found at 2590
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to express PgsBCA complex heterologously in corynebacterium glutamicum, we add Ptac promoter with a short RBS before the start codon and T7 terminater of each protein， respectively.



Source

Bacillus Genome.

References