Reporter

Part:BBa_K3020002:Design

Designed by: WANG WENJIA   Group: iGEM19_BIT   (2019-09-08)
Revision as of 08:17, 8 September 2019 by WANGWENJIA (Talk | contribs) (→‎Source)

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enhanced Green fluorescent protein reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The eGFP gene sequence used in this experiment was obtained from NCBI, sequence ID: 20473140, and the level of foreign gene expression was affected by the preference of codons.The gene sequence was submitted to Jin Weizhi (Suzhou) Bio Co., Ltd. for codon optimization of E. coli host.

References