Regulatory

Part:BBa_K3020001:Design

Designed by: WANG WENJIA   Group: iGEM19_BIT   (2019-09-06)
Revision as of 06:20, 8 September 2019 by WANGWENJIA (Talk | contribs) (→‎References)


recA promoter-Respond to sos response and initiate expression of downstream repair proteins


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The recA promoter fragment was cloned from the genome of E.coli K12 MG1655 strain as a template.Please note that the recA promoter sequences from different genomes are not consistent.

References

Norman A , Hestbjerg Hansen L , Sørensen, Søren J. Construction of a ColD cda Promoter-Based SOS-Green Fluorescent Protein Whole-Cell Biosensor with Higher Sensitivity toward Genotoxic Compounds than Constructs Based on recA, umuDC, or sulA Promoters[J]. Appl Environ Microbiol, 2005, 71(5):2338-2346.