Coding

Part:BBa_K2963004:Design

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-07)
Revision as of 10:42, 7 September 2019 by Registry (Talk | contribs)

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racE - a glutamate racemase from Bacillus sp.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our subject, we get the racE sequenece by PCR of Bacillus genome. When we express the racE protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.


Source

Bacillus Genome.

References