Coding
Part:BBa_K3257034
Designed by: Yi Qiao Group: iGEM19_Fudan-TSI (2019-09-04)
Chloramphenicol Resistant Gene(ChlR)
This part encodes chloramphenicol acetyltransferase which enables E.coli to grow in the environment containing chloramphenicol.
Numbers of different types of colonies grown on plates with different antibiotics
No ChlR | WT ChlR | ChlR_T33X | ChlR_Y92X | ChlR_E97X | ChlR_S121X | ChlR_L158X | ChlR_K182X | ChlR_Q190X | |
---|---|---|---|---|---|---|---|---|---|
Amp | 19 | 192 | 291 | 128 | 236 | 138 | 255 | 175 | 276 |
Amp/Chl | 0 | 30 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |