Coding

Part:BBa_K2933101

Designed by: Dongxu Li   Group: iGEM19_TJUSLS_China   (2019-09-06)
Revision as of 07:24, 7 September 2019 by Dongxu (Talk | contribs)


GST+ recognition site of Prescission Protease+ NDM-23

This part encodes the fusion protein of GST tag and NDM-23 to promote the expression and purification of target protein(NDM-23).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Usage and Biology

This composite part is made up with three basic parts, the GST tag, the cutting site of Prescission Protease and our target protein NDM-23. It encodes a protein which is NDM-23 fused with GST tag. In order to gain the highly purified target protein, we add GST tag in N-terminal of NDM-23 and combine the two parts with the cutting site of Prescission Protease. The fusion protein can be cut off at the cutting site by Prescission Protease. It is convenient for us to purify our target protein.

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Categories
Parameters
None