Coding

Part:BBa_K3257036

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-05)
Revision as of 13:03, 6 September 2019 by YY12138 (Talk | contribs)


Chloramphenicol Resistance Gene Y92X (ChlR_Y92X)

This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 92nd amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None