Coding

Part:BBa_K3257035:Design

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-05)
Revision as of 12:55, 6 September 2019 by YY12138 (Talk | contribs) (→‎Design Notes)


Chloramphenicol Resistance Gene T33X (ChlR_T33X)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the nonsense mutated chloramphenicol resistance gene so that it will not give the host the ability of growing in an environment containing chloramphenicol. We mutated the 99th nucleotide T→G of BBa_K3257034 to make an early stop codon.

Source

Bacteria

References