Coding

Part:BBa_K3257035

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-05)
Revision as of 09:56, 6 September 2019 by YY12138 (Talk | contribs)


Chloramphenicol Resistance Gene T33X (ChlR_T33X)

This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 33rd amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we found that no colony grew on a medium plate containing chloramphenicol, showing its inability of being resistant to chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None