Coding
Part:BBa_K3257031:Design
Designed by: Yi Qiao Group: iGEM19_Fudan-TSI (2019-09-04)
EGFP_Q158X
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is very similar to the wild type EGFP except that it does not give out green light as the wild type EGFP does. We mutate its 472nd nucleotide C→T to make a stop codon at 158th amino acid residual.
Source
BBa_