Part:BBa_K2933016

subclass B1 metallo-beta-lactamase VIM-66, codon optimized in E. coli

This part encodes a protein called VIM-66, which is a metallo-beta-lactamase of subclass B1.

Usage and Biology

Metallo-beta-lactamase VIM was first identified in a patient in Italy who was infected with P. aeruginosa. VIM-66 sequence is mostly similar to VIM-2. Among these VIM variants, VIM-2 appears to be the one most commonly found in the clinic, and VIM-2-expressing bacterial strains have been found in many countries.

In fact, blaVIM genes have been detected mostly in the Mediterranean countries of Europe, in the Far East regions, including Japan, and at present, in American regions, including the U.S. VIM-expressing bacteria have been shown resistant to an array of β-lactam-containing antibiotics.

Due to the high clinical relevance, the widespread and the broad substrate range, VIM-2 is an important drug target to restore the function of carbapenem antibiotics and the treatment of antibiotic resistant bacteria.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 738
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]