Regulatory

Part:BBa_K2992001

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-03)
Revision as of 11:49, 5 September 2019 by YasTen (Talk | contribs)


PntnH

Regulatory region comprising promoter 5'-UTR and RBS for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in Clostridum botulinum.

Usage and Biology

Pntnh<i> is derived from the nerotoxin gene cluster of <i>C. botulinum. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production following food processing.

Characterisation

Data incoming.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None