Coding

Part:BBa_K3257040

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-05)
Revision as of 06:11, 5 September 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Chloramphenicol Resistance Gene K182X (ChlR_K182X)

This part is a mutant of a gene encoding Chl, a resistance gene of chloramphenicol, and it is nonsense mutated at its 182nd amino acid residual. We used this mutated Chl as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None