Conjugation

Part:BBa_K2976010

Designed by: Jiatong Chen   Group: iGEM19_CPU_CHINA   (2019-08-04)
Revision as of 05:00, 5 September 2019 by NF-kB pathway (Talk | contribs) (→‎Usage and Biology)

Improved T2A

Usage and Biology

Oligopeptide “2A‐like” sequences are found in a range of RNA virus genomes controlling protein biogenesis and T2A derives from Thosea asigna virus. The 2A peptide consensus motif is extremely rare and is always associated with cleavage activity between the glycine and the proline. The insertion of T2A within a single open reading frame (ORF) produces multiple proteins. Besides, inserting a GSG linker before the T2A sequence significantly improves its self-cleaving efficiency and ensures complete cleavage. In 2019 CPU_CHINA project, we utilize P2A and T2A to co-express TLR1:TLR2:CD14 cluster on our designer cell membrane.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None