Composite
Part:BBa_K2927063
Designed by: KUO, PEI-CHING Group: iGEM19_CCU_Taiwan (2019-09-04)
T7 promoter-guide RNA-crRNA 3-mutated crRNA 6
The sequence we designed is used for generating crRNA 3 and mutated crRNA 6. CrRNA 3 is used for African swine fever (ASFV) detection. Furthermore, mutated crRNA 6 is the sequence derived from crRNA 6 for negative control to test whether our homemade Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 3 and mutated crRNA 6.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |