Protein_Domain

Part:BBa_K112100:Design

Designed by: Madhvi Venkatesh   Group: iGEM08_UC_Berkeley   (2008-08-14)
Revision as of 02:29, 15 August 2008 by Madhvi (Talk | contribs) (→‎Design Notes)

Cre enzyme without start codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 39
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

PCR mv001/mv002 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112100
-----------------------------------------------
mv001	Forward Biobricking of <Cre!
ccataGAATTCatgAGATCTtccaatttactgaccgtacacc
mv002	Reverse Biobricking of <Cre!
cgttaGGATCCttaatcgccatcttccagc

Source

PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100


References