Part:BBa_K3198003

Xre

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage

It is demonstrated that the res-xre locus from Photorhabdus luminescens and other bacterial species function as bona fide TA modules in Escherichia coli.

It was shown that a RES toxin encoded by the α‐proteobacterium, Sinorhizobium meliloti, is toxic when expressed in Escherichia coli and can be counteracted by its cognate Xre antitoxin.

RES toxin from several bacterial species reversibly inhibits cell growth in E. coli, and that expression of the Xre antitoxin neutralizes this toxicity. However, the cellular function of the RES toxin when activated, as well as structure and the antitoxic mechanism of the Xre antitoxin, remain unknown.

Biology

This part is from the res-xre locus from Photorhabdus luminescens and other bacterial species.

References

Milunovic, B., diCenzo, G.C., Morton, R.A.and Finan, T.M. (2014) Cell growth inhibition upon deletion of four toxin‐antitoxin loci from the megaplasmids of Sinorhizobium meliloti. Journal of Bacteriology, 196, 811–824.

Skjerning, R. B., Senissar, M., Winther, K. S., Gerdes, K., & Brodersen, D. E. (2018). The RES domain toxins of RES-Xre toxin-antitoxin modules induce cell stasis by degrading NAD . Molecular Microbiology, 111(1), 221–236. doi: 10.1111/mmi.14150