DNA

Part:BBa_K2927022

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-08-29)
Revision as of 05:11, 30 August 2019 by Peggy KUO (Talk | contribs)

Negative control sequence for crRNA 3 target

We changed 5 nucleotides of crRNA 1 from 5’ end to make the mutated crRNA 3. It is the sequence for testing whether homemade Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 3 is too short to become the functional protein. There is no concentration for spread out ASFV of our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None