Coding

Part:BBa_K2927004

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-08-28)
Revision as of 04:46, 29 August 2019 by Peggy KUO (Talk | contribs)


Tobaco Etch Virus (TEV) motif

TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. It makes Cas12a protein be collected in the solution. Furthermore, the sequence of the TEV cutting site is used by E.coli.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None