Coding

Part:BBa_K2927004

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-08-28)
Revision as of 04:46, 29 August 2019 by Registry (Talk | contribs)

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Tobaco Etch Virus (TEV) motif

TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. It makes Cas12a protein be collected in the solution. Furthermore, the sequence of the TEV cutting site is used by E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None